Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation
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Poly(hydroxyalkanoate) Production by Cupriavidus necator from Fatty Waste Can Be Enhanced by phaZ1 Inactivation
Although PHAs are regarded as an effective substitute for conventional plastics for a number of medical and agricultural applications1 and food packaging2, their full-scale manufacturing is hampered by high production costs3. Factors affecting the cost of PHAs include raw materials availability, suitable process design, and downstream processing4,5. Since almost 50 % of the total production cos...
متن کاملPolyhydroxyalkanoates production by engineered Cupriavidus necator from waste material containing lactose.
Cupriavidus necator DSM 545 is a well-known polyhydroxyalkanoates (PHAs) producer, but unable to grow on lactose. The aim of this study was to construct a recombinant strain of C. necator that can use lactose-containing waste material such as cheese whey, to produce PHAs. One of the intracellular PHA depolymerases (phaZ1) of C. necator was chosen to insert the lacZ, lacI and lacO genes of Esche...
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Background and Aims: Gold nanoparticles have potential applications in the areas of medicine, target drug delivery, cancer diagnosis and therapy, electronic, etc. Recently, biological system is considered as an environmental friendly method for synthesis of stable nanoparticles. Methods: We demonstrated a biological system for formation of stable gold nanoparticle by using Cupriavidus necator w...
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We investigated the effects of different feeding regimens (1-pulse, stepwise, and continuous) of fermented food-waste liquid on polyhydroxybutyrate (PHB) production. The fermentation liquid was filtered with a membrane filter (pore size, 0.45 μm) to remove anaerobic microorganisms and solids and used as a carbon source for Cupriavidus necator. One-pulse feeding yielded the highest cell concentr...
متن کاملFactors Affecting Poly(3-hydroxybutyrate) Production from Oil Palm Frond Juice by Cupriavidus necator (CCUG52238T)
Factors influencing poly(3-hydroxybutyrate) P(3HB) production by Cupriavidus necator CCUG52238(T) utilizing oil palm frond (OPF) juice were clarified in this study. Effects of initial medium pH, agitation speed, and ammonium sulfate (NH(4))(2)SO(4) concentration on the production of P(3HB) were investigated in shake flasks experiments using OPF juice as the sole carbon source. The highest P(3HB...
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ژورنال
عنوان ژورنال: Chemical and Biochemical Engineering Quarterly Journal
سال: 2015
ISSN: 0352-9568,1846-5153
DOI: 10.15255/cabeq.2014.2248